Sorry for this late reply. As no one replied on this, I would like to share my view on this. I am not sure if Twain supports to read barcode in .net at that time. But it seems that it is ok now. If it does not support to do this, you can also try some other ways, like employing a third party control (.net barcode reader) to read the barcode in .net. You can just add the tool your program and do a few steps to finish the barcode reading. Many tools support to read many barcode types, including QR Code, PDF417, Code 39, Code 128, etc. Hope this would be helpful for others.